DNA

Part:BBa_K4889003:Design

Designed by: zhaoxia zhang   Group: iGEM23_Shanghai-MedX   (2023-09-06)


MST2 (mutant)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 12
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


Design Notes

Since the three sites to be mutated are genetically close together, we synthesized the mutated base sequences by designing primers. Subsequently, we obtained MST2 1-308 (mutant) by PCR.


Source

MST2 1-308 (mutant) from BBa_K4889000.

References